BioMin 5-strain probioticum

biomin_moleculeThe animal production in the EU faces a difficult situation, due to the total ban of antibiotic growth promoters (AGPs). Competitive exclusion (CE) has been shown to be an effective means of protecting birds against Salmonella. Numerous intestinal bacteria were isolated out of the gut of several healthy birds and thoroughly characterized combining morphological, physiological and genotypic methods. The most promising strains were evaluated for important probiotic criteria such as adhesion capability to intestinal cell lines, inhibition of pathogens, (e.g. S. enteritidis, S. typhimurium, S. choleraesuis, C. jejuni, E. coli and Cl. perfringens), immunological activity, range of metabolic end products like organic acids, fermentation performance, stability against acids and bile salts, storage stability and safety status.

Based on these results a product consisting of 5 well-defined strains belonging to the genera Enterococcus, Pediococcus, Lactobacillus and Bifidobacterium (Enterococcus faecium, Bifidobacterium animalis, Pediococcus acidilactici, Lactobacillus salivarius, Lactobacillus reuteri) has been designed.

In the course of a feeding trial the effect of the developed defined CE product on cecal colonization of
S. enteritidis in birds was investigated. Day-old chicks were randomly allocated to two groups (10 birds per group) and reared in cages at isolation units. The control group was fed a standard diet, the experimental group received additionally the CEproduct (Avian Biomin probioticum) added to the drinking water on day 1, 2, and 3. Food and water were given ad libitum. Birds were orally inoculated with 0.1 ml S. enteritidis (SE NalrSpcr) culture containing 1.0 x 106 CFU/ml at age of 3 days. At day five, three birds out of each group were sacrificed and their cecal contents were cultured for S. enteritidis using the method of Barrow (1987). In the control group an average S. enteritidis viable count of 3.62 cfu log 10 per g cecal content was found whereas in the experimental group the average number of S. enteritidis in the cecal content was significantly reduced below the detection limit (P<0.05). These developments lead to a situation with a demand for alternatives, which can help to counteract possible problems like increased occurrence of infectious disease, performance losses in bird husbandry and the increased use of therapeutic antibiotics.

This is a problem particularly affecting the bird keeper as a result of the possible transfer of pathogens (Salmonella, E.coli, Campylobacter). Newly hatched chicks of the modern birds husbandry lack development of the intestinal microflora, and as a consequence birds are particularly susceptible to pathogen colonization at a very young age. Feeding of adult microflora (intestinal contents) protects newly hatched chicks from Salmonella infections via establishment of an improved intestinal microflora. Competitive exclusion (CE) strategies and the use of specific probiotics have been shown to be an effective means of manipulating or managing the composition of the microbial population in the gastrointestinal tract of birds and thus protecting birds from infections with pathogenic bacteria. In particular the CE concept is able to increase colonization resistance of day-old chicks against Salmonella, which is the most important bacterial contaminant transmitted by birds. It causes food borne diseases and heavy losses to the birds. Therefore the bird keepers should be provided with alternatives to antibiotics to ensure healthy breeding.

In the course of a feeding trial the effect of the developed defined CE-product on cecal colonization of S. enteritidis in broiler chickens was investigated. The findings observed in this experiment suggest that the defined CEproduct Avian Biomin probioticum applied via the drinking water may be beneficial in the control of intestinal colonization of chickens by Salmonella Enteritidis.